Les automates d’immuno-analyse pour la recherche et le dosage d’immunoglobulines E spécifiques unitaires

  title={Les automates d’immuno-analyse pour la recherche et le dosage d’immunoglobulines E sp{\'e}cifiques unitaires},
  author={C. Klingebiel and Romain Philippe and Philippe Mathieu and J. Vitte and P.A. Apoil},
  journal={Revue Française d'Allergologie},



Distinct differences in analytical performance of two commercially available assays for specific IgE to egg white and house dust mite allergens

In this evaluation of analytical performance of two widely used assay systems, ImmunoCAP showed higher accuracy in quantitation of specific IgE and greater resistance against competing allergen-specific non-IgE antibodies which may arise through natural or dietary exposure, or as a result ofallergen immunotherapy treatment.

Agreement Between Predictive, Allergen-Specific IgE Values Assessed by ImmunoCAP and IMMULITE 2000 3gAllergy™ Assay Systems for Milk and Wheat Allergies

The 2 systems may be interchangeable for diagnosis of milk and wheat allergies in young children and show good agreement between the methods in the diagnosis of egg allergy.

Advances in IgE testing for Diagnosis of Allergic Disease.

Validation of the analytical performance of the NOVEOS™ System, a system which improves upon the third-generation in vitro allergy testing technology

A good precision and linearity as well as a detection limit <0.1 kU/L were observed, with minimal impact of common interfering substances on patient recoveries, in the NOVEOS allergen sIgE testing system.

Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components

The risk of CCD interference that compromises quantitative IgE results can be mitigated by the addition of a soluble CCD inhibitor to positive CCD-sIgE containing sera or by alternatively using a non-cellulose based sIg E assay, such as the NOVEOS assay.

Quality management in IgE-based allergy diagnostics

Assays for total and allergen-specific IgE are essential serological tests in the diagnostic work-up of immediate type hypersensitivity reactions and atopic diseases and future challenges include a more valid quantification of sIgE which would allow true comparisons with the international units for total IgE, and the use of harmonizedallergen reagents for the most important allergenic sources, which have hampered inter-assay comparability in the past.

Comparison of allergen-specific IgE levels between Immulite 2000 and ImmunoCAP systems against six inhalant allergens and ten food allergens

Immulite 2000 and ImmunoCAP assays demonstrated good concordance and correlation for 16 common allergens, but international standards against each specific allergen for calibration and harmonization of sIgE tests are still needed.