Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires.

@article{Schreier2009LeptospirosisRF,
  title={Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires.},
  author={Stefan Schreier and Wannapong Triampo and Galayanee Doungchawee and Darapond Triampo and Sudarat Chadsuthi},
  journal={Biological research},
  year={2009},
  volume={42 1},
  pages={5-12}
}
Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for… CONTINUE READING

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