Leptin levels in human and rodent: Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects

@article{Maffei1995LeptinLI,
  title={Leptin levels in human and rodent: Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects},
  author={Margherita Maffei and Jeffrey L. Halaas and Eric Ravussin and Richard E. Pratley and G H Lee and Y Zhang and Hong Fei and S. Kim and Roger L. Lallone and Subramanian Ranganathan and Philip A. Kern and Jeffrey M. Friedman},
  journal={Nature Medicine},
  year={1995},
  volume={1},
  pages={1155-1161}
}
Leptin, the gene product of the obese gene, may play an important role in regulating body weight by signalling the size of the adipose tissue mass. Plasma leptin was found to be highly correlated with body mass index (BMI) in rodents and in 87 lean and obese humans. In humans, there was variability in plasma leptin at each BMI suggesting that there are differences in its secretion rate from fat. Weight loss due to food restriction was associated with a decrease in plasma leptin in samples from… 
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References

SHOWING 1-10 OF 68 REFERENCES
Effects of the obese gene product on body weight regulation in ob/ob mice.
TLDR
The data suggest that the OB protein regulates body weight and fat deposition through effects on metabolism and appetite.
The expression of tumor necrosis factor in human adipose tissue. Regulation by obesity, weight loss, and relationship to lipoprotein lipase.
TLDR
The data suggest that endogenous TNF expression in adipose tissue may help limit obesity in some subjects, perhaps by increasing insulin resistance and decreasing LPL.
The effects of weight loss on the activity and expression of adipose-tissue lipoprotein lipase in very obese humans.
TLDR
It is concluded that weight loss in very obese subjects leads to the increased activity and expression of lipoprotein lipase, thereby potentially enhancing lipid storage and making further weight loss more difficult.
Overexpression of the obese (ob) gene in adipose tissue of human obese subjects
TLDR
Investigation of human ob expression in subcutaneous and omental adipose tissue obtained from non–obese and massively obese subjects using in situ hybridization histochemistry and report on overexpression in obese people.
Weight-reducing effects of the plasma protein encoded by the obese gene.
TLDR
Injection of wild-type mice twice daily with the mouse protein resulted in a sustained 12 percent weight loss, decreased food intake, and a reduction of body fat from 12.2 to 0.7 percent, suggesting that the OB protein serves an endocrine function to regulate body fat stores.
Increased obese mRNA expression in omental fat cells from massively obese humans
TLDR
Ex vivo obese mRNA expression is elevated in ex vivo omental adipocytes isolated from massively obese humans in the absence of an identifiable mutation, and it is speculated that this increased expression may suggest that the massively obese are insensitive to the putative regulatory function(s) of the obese gene product.
Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neural networks.
TLDR
The behavioral effects after brain administration suggest that OB protein can act directly on neuronal networks that control feeding and energy balance in ob/ob and diet-induced obese mice.
Increased expression in adipocytes of ob RNA in mice with lesions of the hypothalamus and with mutations at the db locus.
  • M. MaffeiH. Fei J. Friedman
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1995
TLDR
In db/db and lesioned mice, quantitative differences in expression level of ob RNA correlated with adipocyte lipid content, suggesting that both the db gene and the hypothalamus are downstream of the ob gene in the pathway that regulates adipose tissue mass.
Enhanced expression of rat obese (ob) gene in adipose tissues of ventromedial hypothalamus (VMH)-lesioned rats.
TLDR
In VMH-lesioned rats, the abundance of ob mRNA increased after the gain of body weight and marked expression was observed on 15th day after making VMH lesion, suggesting that ob gene might be up-regulated with fat accumulation even in non-genetically obese animals.
Appetite and the regulation of body composition
  • D. Weigle
  • Medicine
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1994
TLDR
The bulk of evidence suggests that the plasma level of one or more currently unidentified stable circulating molecules increases in proportion to total body fat content and augments the effect of meal‐related satiety signals in the central nervous system.
...
...