Lepidopteran cell variants resistant to 5-bromodeoxyuridine and their use for transfection of the HSV-TK gene.

@article{Xie1991LepidopteranCV,
  title={Lepidopteran cell variants resistant to 5-bromodeoxyuridine and their use for transfection of the HSV-TK gene.},
  author={Wei Xie and Dacheng He and Xun Z Wang and Qin X Long and Zhixiao Pu},
  journal={Journal of cell science},
  year={1991},
  volume={100 ( Pt 1)},
  pages={243-7}
}
Following mutagenesis of cultured lepidopteran cells (Spodoptera frugiperda) by ethylmethanesulfonate, three variants resistant to 5-bromodeoxyuridine (BrdUrd) were isolated. These clones were 100- to 200-fold more resistant to BrdUrd than the parental cells and were shown to be deficient in thymidine kinase (TK). The drug-resistant phenotype was stable for up to three years of culture under nonselective conditions. It was also found that the S. frugiperda cell line was highly resistant to… CONTINUE READING

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