Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cells.

Abstract

Currently, lentiviral vectors for research and gene therapy are produced from 293-T cells that are transiently transfected with plasmids encoding the vector and helper functions. However, transiently transfected vectors as well as the presence of SV40 virus large T-antigen (T-Ag) cause serious technical and safety considerations. We aimed to exploit single… (More)
DOI: 10.1089/hum.2010.143

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@article{GamaNorton2011LentivirusPI, title={Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cells.}, author={Leonor Gama-Norton and Lacramioara Botezatu and Sabrina Herrmann and Matthias Schweizer and Paula M. Alves and H. Hauser and Dagmar Wirth}, journal={Human gene therapy}, year={2011}, volume={22 10}, pages={1269-79} }