Lentivirus gene transfer in murine hematopoietic progenitor cells is compromised by a delay in proviral integration and results in transduction mosaicism and heterogeneous gene expression in progeny cells.

@article{Mikkola2000LentivirusGT,
  title={Lentivirus gene transfer in murine hematopoietic progenitor cells is compromised by a delay in proviral integration and results in transduction mosaicism and heterogeneous gene expression in progeny cells.},
  author={Hanna Mikkola and N B Woods and Marketa Sj{\"o}gren and Hildur B Helgadottir and Isao Hamaguchi and Sten Erik W Jacobsen and Didier Trono and Stefan Karlsson},
  journal={Journal of virology},
  year={2000},
  volume={74 24},
  pages={11911-8}
}
Human immunodeficiency virus type 1-based lentivirus vectors containing the green fluorescent protein (GFP) gene were used to transduce murine Lin(-) c-kit(+) Sca1(+) primitive hematopoietic progenitor cells. Following transduction, the cells were plated into hematopoietic progenitor cell assays in methylcellulose and the colonies were scored for GFP positivity. After incubation for 20 h, lentivirus vectors transduced 27.3% +/- 6.7% of the colonies derived from unstimulated target cells, but… CONTINUE READING

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