Lentiviral vectors for efficient transduction of isolated primary quiescent hepatocytes.

BACKGROUND/AIMS Lentiviral vectors were designed to obtain efficient transduction of primary quiescent hepatocytes. METHODS A hepatitis B virus (HBV) fragment containing enhancers and posttranscriptional regulatory element was used to increase expression levels. The human immunodeficiency virus (HIV) central polypurine tract (PPT) was used to increase… CONTINUE READING