Lentiviral Transfection with the PDGF-B Gene Improves Diabetic Wound Healing

@article{Lee2005LentiviralTW,
  title={Lentiviral Transfection with the PDGF-B Gene Improves Diabetic Wound Healing},
  author={James A. Lee and J. Alejandro Conejero and James M. Mason and Brian M. Parrett and Kelly D Wear-Maggitti and Robert T. Grant and Arnold S. Breitbart},
  journal={Plastic and Reconstructive Surgery},
  year={2005},
  volume={116},
  pages={532-538}
}
Background: The treatment of diabetic wounds remains a difficult challenge. The present study investigates whether platelet-derived growth factor (PDGF) lentiviral gene therapy can improve diabetic wound healing in the diabetic db/dbmouse. Methods: PDGF cDNA was cloned and lentiviral vectors were constructed with either the PDGF-B or green fluorescence protein (GFP) gene. A 2 × 2-cm full-thickness dermal wound was made on each db/db mouse. Animals were divided into three groups, with eight… 
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A new model of delayed wound healing in the pig which mimicks deficiencies seen in diabetic wounds is demonstrated and is able to accelerate wound healing by using cell therapy and further improved it using gene therapy.
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