Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability.

@article{CudrMauroux2003LentivectormediatedTO,
  title={Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability.},
  author={Christophe Cudr{\'e}-Mauroux and Teresa Occhiodoro and St{\'e}phane K{\"o}nig and Patrick Salmon and Laurent Bernheim and Didier Trono},
  journal={Human gene therapy},
  year={2003},
  volume={14 16},
  pages={1525-33}
}
Conditionally immortalized human cells are valuable substrates for basic biologic studies, as well as for the production of specific proteins and for the creation of bioartificial organs. We previously demonstrated that the lentivector-mediated transduction of immortalizing genes into human primary cells is an efficient method for obtaining such cell lines. Here, we used human muscle satellite cells as model targets to examine the impact of the transduced genes on the genotypic and phenotypic… CONTINUE READING