Lens proteomics: analysis of rat crystallin sequences and two-dimensional electrophoresis map.

@article{Lampi2002LensPA,
  title={Lens proteomics: analysis of rat crystallin sequences and two-dimensional electrophoresis map.},
  author={Kirsten J. Lampi and Marjorie Shih and Yoji Ueda and Thomas R. Shearer and Larry L. David},
  journal={Investigative ophthalmology & visual science},
  year={2002},
  volume={43 1},
  pages={216-24}
}
PURPOSE To determine the sequence of four rat beta-crystallins, confirm the sequences by mass spectrometry, and produce a two-dimensional electrophoresis (2-DE) map of soluble crystallins in young rat lens. METHODS New or additional sequences were determined for betaB1, betaB3, betaA3, and betaA4-crystallin cDNAs from Sprague-Dawley rats, and the deduced protein sequences confirmed by mass spectrometry. The identity and relative abundance of each crystallin was then determined by 2-DE of… CONTINUE READING

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