Length-independent separation of DNA restriction fragments in two-dimensional gel electrophoresis

@article{Fischer1979LengthindependentSO,
  title={Length-independent separation of DNA restriction fragments in two-dimensional gel electrophoresis},
  author={S. Fischer and L. Lerman},
  journal={Cell},
  year={1979},
  volume={16},
  pages={191-200}
}
  • S. Fischer, L. Lerman
  • Published 1979
  • Biology, Medicine
  • Cell
  • When double helical DNA is exposed to conditions favoring partial melting in polyacrylamide gels, its electrophoretic mobility undergoes a sharp cooperative transition, resulting in a large reduction in mobility. In the present experiments, where the transition is effected at a uniform temperature of 60 degrees C in a concentration gradient of a urea-formamide mixture, each Eco RI fragment of lambda or E. coli DNA exhibits the mobility transition at a characteristic concentration of the… CONTINUE READING
    334 Citations
    Two-dimensional electrophoretic separation of restriction enzyme fragments of DNA.
    • 28
    Denaturing gradient gel electrophoresis to detect methylation changes in DNA.
    • M. Shiraishi
    • Chemistry, Medicine
    • Methods in molecular biology
    • 2004
    • 2
    Separation of branched from linear DNA by two-dimensional gel electrophoresis.
    • 107
    Denaturing gradient gel electrophoretic analysis of minisatellite alleles
    • 15

    References

    SHOWING 1-10 OF 22 REFERENCES
    Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs.
    • 109
    A two-dimensional electrophoretic procedure for the separation of DNA restriction fragments.
    • 11
    Polyacrylamide Gel Electrophoresis
    • 636
    • PDF
    Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    • 551
    High resolution two-dimensional electrophoresis of proteins.
    • P. O'Farrell
    • Chemistry, Medicine
    • The Journal of biological chemistry
    • 1975
    • 16,295
    • PDF
    Optical studies of a conformational change in DNA before melting.
    • 102
    Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    • 1,576
    • PDF
    Multidimensional restriction enzyme analysis of complex genomes.
    • 20