Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection.

@article{Mykov2008LeishmaniaIS,
  title={Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection.},
  author={Jitka My{\vs}kov{\'a} and Jan Vot{\'y}pka and Petr Volf},
  journal={Journal of medical entomology},
  year={2008},
  volume={45 1},
  pages={133-8}
}
Quantification of Leishmania parasites in the sand fly digestive tract is important for evaluation of vector competence. We compared quantitative polymerase chain reaction (Q-PCR) with two "traditional" methods, estimation in situ and direct counting with the aid of a hemocytometer, to evaluate their usefulness in different parasite-vector combinations. Phlebotomus duboscqi Neveu-Lemarie and Phlebotomus arabicus Theodor sand flies were infected with Leishmania major and Leishmania infantum… CONTINUE READING
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