Leish-KIT, a stable direct agglutination test based on freeze-dried antigen for serodiagnosis of visceral leishmaniasis.

@article{Meredith1995LeishKITAS,
  title={Leish-KIT, a stable direct agglutination test based on freeze-dried antigen for serodiagnosis of visceral leishmaniasis.},
  author={S. Meredith and N. Kroon and E. Sondorp and J. Seaman and M. Goris and C. V. van Ingen and H. Oosting and G. Schoone and W. J. Terpstra and L. Oskam},
  journal={Journal of clinical microbiology},
  year={1995},
  volume={33 7},
  pages={
          1742-5
        }
}
In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based on this freeze-dried antigen. It was shown that the freeze-dried antigen remains fully active, even after storage at 56 degrees C for 18 months. With a cutoff value of 1:1,600, the… Expand
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