Legacy of a Distinguished Scientist: George E. Palade

  title={Legacy of a Distinguished Scientist: George E. Palade},
  author={Manfred Vincenz Singer},
  pages={518 - 519}
  • M. Singer
  • Published 12 February 2004
  • Medicine
  • Pancreatology

The neuronal porosome complex in health and disease

Atomic force microscopy and small-angle X-ray solution scattering studies have provided three-dimensional structural details of the native neuronal porosome at sub-nanometer resolution, providing insights into the molecular mechanism of its function.

Cell secretion and membrane fusion: highly significant phenomena in the life of a cell

This paper aims to demonstrate the efforts towards in-situ applicability of EMMARM, as to provide real-time information about the immune responses of mice to certain types of chemotherapy.

Porosome in the Exocrine Pancreas: A Detailed EM Study suppressor

This study carefully examined using electron microscopy, the detailed structure of the porosome complex in acinar cells of the exocrine pancreas, and demonstrates for the first time the docking of a single secretory vesicle or zymogen granule at the base of more than one porosomes complex.


For the first time, in cellular ultrastructure and physiology, he described the mitochondria, nervous fibers of C type, interneuronal and neuromuscular synapses, the ribosomes (Palade granules), the role of rough endoplasmic reticullum in protein synthesis, the atrial specific granules, the messangial cells from renal corpuscles, the different types of intercellular junctions.

Porosome: the secretory NanoMachine in cells.

  • B. Jena
  • Biology
    Methods in molecular biology
  • 2013
The discovery of permanent secretory portals or nanomachines at the cell plasma membrane called POROSOMES, where membrane-bound secretory vesicles transiently dock and fuse to release intravesicular contents to the cell exterior, has provided a molecular understanding of cell secretion.

Porosome: the secretory portal

  • B. Jena
  • Biology
    Experimental biology and medicine
  • 2012
The composition of the porosome, its dynamics, its structure at nanometer resolution in realtime using atomic force and electron microscopy, and its functional reconstitution into artificial lipid membrane, has resulted in a paradigm shift and a molecular understanding of the secretory process in cells.

3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex

The results demonstrate the power and scope of the combined engagement of EM and AFM imaging of fixed semi-dry cells, capable of providing a wealth of new information on cellular structure–function and interactions.

NanoCellBiology of Secretion

  • B. Jena
  • Biology
    SpringerBriefs in Biological Imaging
  • 2012
The fusion of membrane-bound secretory vesicles at the porosome base, and the molecular mechanism of secretORY vesicle swelling required for content expulsion during cell secretion, is discussed in this Springer Brief.

Porosome: the secretory portal in cells.

The discovery of the porosome complex and the molecular mechanism of its function and how this information provides a new understanding of cell secretion are discussed.



Scanning Probe Evolution in Biology

Instruments with increased imaging rates, lower probe-specimen force interactions, and probe configurations not constrained to planar surfaces are being developed, with the goal of imaging processes at the single-molecule level—not only at surfaces but also within three-dimensional volumes—in real time.