Lectin precipitation using phytohemagglutinin-L(4) coupled to avidin-agarose for serological biomarker discovery in colorectal cancer.

@article{Kim2008LectinPU,
  title={Lectin precipitation using phytohemagglutinin-L(4) coupled to avidin-agarose for serological biomarker discovery in colorectal cancer.},
  author={Yong-Sam Kim and Ok Lye Son and Ju Yeon Lee and Sun Ran Kim and Sejeong Oh and Yoon Suk Lee and C -W Kim and Jong Shin Yoo and Jeong-hwa Lee and Eiji Miyoshi and Naoyuki Taniguchi and Samir M Hanash and Hyang Sook Yoo and Jeong Heon Ko},
  journal={Proteomics},
  year={2008},
  volume={8 16},
  pages={3229-35}
}
N-acetylglucosaminyltransferase V (GnT-V) has been reported to be upregulated in malignant cancer cells, and its targets have been sought after with regard to biomarker identification. The low capacity and high false positive rates of 2-DE gel-based lectin blots using phytohemagglutinin-L(4) (L-PHA) prompted us to develop a novel protocol for identifying GnT-V targets, in which serum proteins were subjected to immunodepletion, alkylation, and lectin precipitation using L-PHA coupled to avidin… CONTINUE READING
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