Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies.

@article{Carlos2002LectinIA,
  title={Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies.},
  author={Alejandro de Carlos and Amado Caride-Castro and Vicenta Soledad Mart{\'i}nez-Zorzano and Mar{\'i}a P{\'a}ez de la Cadena and Francisco Javier Rodr{\'i}guez-Berrocal},
  journal={International journal of oncology},
  year={2002},
  volume={20 2},
  pages={367-72}
}
A method to improve the reactivity to specific lectins of N-glycoproteins isolated from human colorectal mucosa and from adenocarcinoma biopsies was developed using a combination of techniques. Total protein extracts were subjected to affinity chromatography, using the immobilised lectin Concanavalin A coupled to Sepharose, by fast performance liquid… CONTINUE READING