Lectin-biotin assay for slime present inin situ biofilm produced byStaphylococcus epidermidis using transmission electron microscopy (TEM)

  title={Lectin-biotin assay for slime present inin situ biofilm produced byStaphylococcus epidermidis using transmission electron microscopy (TEM)},
  author={B. Sanford and V. Thomas and S. Mattingly and M. A. Ramsay and M. M. Miller},
  journal={Journal of Industrial Microbiology},
A lectin-biotin assay was developed for use in the specific detection of slime produced byStaphylococcus epidermidis RP62A and M187sp11 grown in a chemically defined medium. Mature biofilm was formed on polyvinylchloride (PVC) disks using a combined chemostat-modified Robbins device (MRD) model system. Specimens fixedin situ were: 1) stained with ruthenium red; 2) reacted overnight with biotin-labeled lectins (WGA, succinyl-WGA, Con A, or APA) followed by treatment with gold-labeled extravidin… Expand
A dual fluorescence technique for visualization ofStaphylococcus epidermidis biofilm using scanning confocal laser microscopy
A new dual fluorescence technique is described which, when combined with scanning confocal laser microscopy (SCLM), is potentially useful as a specific noninvasive tool for studying the effect of antimicrobial agents on the process of biofilm formation and for the characterization of the architecture of S. epidermidis biofilm. Expand
[Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria].
It is shown that lectins labeled with colloidal gold could be used as an express method for the identification and localization of carbohydrates in glycopolymers of the biofilm exopolymeric matrix. Expand
Glycomics Microarrays Reveal Differential In Situ Presentation of the Biofilm Polysaccharide Poly-N-acetylglucosamine on Acinetobacter baumannii and Staphylococcus aureus Cell Surfaces
Differences in PNAG presentation and accessibility for interactions on Gram-positive and Gram-negative cell surfaces which may play an important role in biofilm-mediated pathogenesis are indicated. Expand
Application of fluorescently labelled lectins for the study of polysaccharides in biofilms with a focus on biofouling of nanofiltration membranes
The biofilm state is the dominant microbial lifestyle in nature. A biofilm can be defined as cells organised as microcolonies embedded in an organic polymer matrix of microbial origin living at anExpand
Spontaneous switch to PIA-independent biofilm formation in an ica-positive Staphylococcus epidermidis isolate.
The combined data indicate that biofilm formation in S. epidermidis is obviously ensured by more than one mechanism suggesting that this life style represents a crucial factor for this organism. Expand
A new enzymatic method for the detachment of particle associated soil bacteria.
A new enzymatic technique for the detachment of bacteria from soil particles was developed and applied to different soil samples taken at various sampling sites and depths, revealing up to 22-fold higher total cell counts for all investigated soil samples compared to the conventional detachment method, a simple dispersion with sodium pyrophosphate buffer. Expand
Adhesion of Aspergillus niger and Penicillium expansumspores on Fez cedar wood substrata
It was found that spores surface were hydrophilic, strongly electron donating and weakly electron accepting using contact angle measurements, while wood surface was hydrophobic, exhibits a character relatively more electron-donor than electron-acceptor. Expand
Hyperosmotic Infusion and Oxidized Surfaces Are Essential for Biofilm Formation of Staphylococcus capitis From the Neonatal Intensive Care Unit
Advice is provided in regard to the preparation of hyperosmolar total parenteral nutrition and the engineering of CVC surfaces that can minimize the risk of catheter-related bloodstream infections caused by S. capitis in the NICU. Expand
Procedure to Quantify Biofilm Activity on Carriers used in Wastewater Treatment Systems
A procedure is presented for evaluating and comparing the biological activity of biofilms attached to various biofilm carriers by measurement of the glucose consumption rate. This technique allowsExpand
Significance of Polysaccharides in Microbial Physiology and the Ecology of Hydrothermal Vent Environments
Genome sequence analysis of several hyperthermophiles indicates that the enzymatic machinery to synthesize and hydrolyze polysaccharides is present in this group of microorganisms, supported by the biochemical characteristics of glycosidases from hyperthernophiles in addition to the observation that several hyperThermophile form biofilms in pure and co-culture. Expand


Investigation on extracellular slime substance produced by Staphylococcus epidermidis.
  • A. Ludwicka, G. Uhlenbruck, +4 authors G. Pulverer
  • Biology, Medicine
  • Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology
  • 1984
Production of the extracellular slime substance was completely inhibited by subinhibitory concentrations of the glycosylation inhibitor tunicamycin, whereas penicillin had no influence. Expand
Identification of an antigenic marker of slime production for Staphylococcus epidermidis
Kinetic studies suggested that SAA is a marker for surface accumulation whereas CPA mediates initial adherence, and chemical analysis of partially purified SAA found SAA to be glucose rich and galactose poor, and chemically distinguished SAA from CPA. Expand
Isolation and characterization of a novel 20-kDa sulfated polysaccharide from the extracellular slime layer of Staphylococcus epidermidis.
The crude slime product derived from two reference Staphylococcus epidermidis strains and two clinical isolates was found to contain protein, and the presence of one species of low-sulfated polysaccharide with a relative molecular mass of 20-kDa was revealed. Expand
Isolation and composition of the extracellular slime made by coagulase-negative staphylococci in a chemically defined medium.
Slime isolated after growth of four strains of coagulase-negative staphylococci on chemically defined medium plus agar was rich in galactose. However, when sterile agar plates were extracted withExpand
Staphylococcal slime: a cautionary tale
The occurrence of D- and L-galactose and pyruvate and sulfate residues and methylation analysis of these crude slime preparations showed that the agar was the main source of crude slime, suggesting that the preparation was largely an artifact of the growth and isolation procedures. Expand
Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces
The results suggest that slime-mediated adherence may be a critical factor in the pathogenesis of S. epidermidis infections of medical devices. Expand
Isolation and characterization of a capsular polysaccharide adhesin from Staphylococcus epidermidis.
It is concluded that the capsular polysaccharide of some strains of coagulase-negative staphylococci is an important factor in adherence to catheter tubing. Expand
Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action
  • J. Luft
  • Chemistry, Medicine
  • The Anatomical record
  • 1971
The results indicate that ruthenium red, as a hexavalent cation, precipitates a large variety of polyanions by ionic interaction, and that its classical reaction with pectin is typical rather than specific. Expand
Study of the interaction of concanavalin A with staphylocccal teichoic acids.
The usefulness of the interaction of Concanavalin A with teichoic acids in studying the immunochemistry of these antigens was demonstrated. Expand
Isolation and characterization of transposon mutants of Staphylococcus epidermidis deficient in capsular polysaccharide/adhesin and slime
Results support previous findings indicating that in S. epidermidis PS/A is involved with in vitro adherence to plastic biomaterials and elaboration of PS/ a is closely associated with slime production. Expand