Lectin-biotin assay for slime present inin situ biofilm produced byStaphylococcus epidermidis using transmission electron microscopy (TEM)

@article{Sanford2005LectinbiotinAF,
  title={Lectin-biotin assay for slime present inin situ biofilm produced byStaphylococcus epidermidis using transmission electron microscopy (TEM)},
  author={B. Sanford and V. Thomas and S. Mattingly and M. A. Ramsay and M. M. Miller},
  journal={Journal of Industrial Microbiology},
  year={2005},
  volume={15},
  pages={156-161}
}
A lectin-biotin assay was developed for use in the specific detection of slime produced byStaphylococcus epidermidis RP62A and M187sp11 grown in a chemically defined medium. Mature biofilm was formed on polyvinylchloride (PVC) disks using a combined chemostat-modified Robbins device (MRD) model system. Specimens fixedin situ were: 1) stained with ruthenium red; 2) reacted overnight with biotin-labeled lectins (WGA, succinyl-WGA, Con A, or APA) followed by treatment with gold-labeled extravidin… Expand
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References

SHOWING 1-10 OF 28 REFERENCES
Investigation on extracellular slime substance produced by Staphylococcus epidermidis.
  • A. Ludwicka, G. Uhlenbruck, +4 authors G. Pulverer
  • Biology, Medicine
  • Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology
  • 1984
TLDR
Production of the extracellular slime substance was completely inhibited by subinhibitory concentrations of the glycosylation inhibitor tunicamycin, whereas penicillin had no influence. Expand
Identification of an antigenic marker of slime production for Staphylococcus epidermidis
TLDR
Kinetic studies suggested that SAA is a marker for surface accumulation whereas CPA mediates initial adherence, and chemical analysis of partially purified SAA found SAA to be glucose rich and galactose poor, and chemically distinguished SAA from CPA. Expand
Isolation and characterization of a novel 20-kDa sulfated polysaccharide from the extracellular slime layer of Staphylococcus epidermidis.
TLDR
The crude slime product derived from two reference Staphylococcus epidermidis strains and two clinical isolates was found to contain protein, and the presence of one species of low-sulfated polysaccharide with a relative molecular mass of 20-kDa was revealed. Expand
Isolation and composition of the extracellular slime made by coagulase-negative staphylococci in a chemically defined medium.
Slime isolated after growth of four strains of coagulase-negative staphylococci on chemically defined medium plus agar was rich in galactose. However, when sterile agar plates were extracted withExpand
Staphylococcal slime: a cautionary tale
TLDR
The occurrence of D- and L-galactose and pyruvate and sulfate residues and methylation analysis of these crude slime preparations showed that the agar was the main source of crude slime, suggesting that the preparation was largely an artifact of the growth and isolation procedures. Expand
Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces
TLDR
The results suggest that slime-mediated adherence may be a critical factor in the pathogenesis of S. epidermidis infections of medical devices. Expand
Isolation and characterization of a capsular polysaccharide adhesin from Staphylococcus epidermidis.
TLDR
It is concluded that the capsular polysaccharide of some strains of coagulase-negative staphylococci is an important factor in adherence to catheter tubing. Expand
Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action
  • J. Luft
  • Chemistry, Medicine
  • The Anatomical record
  • 1971
TLDR
The results indicate that ruthenium red, as a hexavalent cation, precipitates a large variety of polyanions by ionic interaction, and that its classical reaction with pectin is typical rather than specific. Expand
Study of the interaction of concanavalin A with staphylocccal teichoic acids.
TLDR
The usefulness of the interaction of Concanavalin A with teichoic acids in studying the immunochemistry of these antigens was demonstrated. Expand
Isolation and characterization of transposon mutants of Staphylococcus epidermidis deficient in capsular polysaccharide/adhesin and slime
TLDR
Results support previous findings indicating that in S. epidermidis PS/A is involved with in vitro adherence to plastic biomaterials and elaboration of PS/ a is closely associated with slime production. Expand
...
1
2
3
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