Lectin affinity chromatography.

  title={Lectin affinity chromatography.},
  author={Hudson H. Freeze},
  journal={Current protocols in protein science},
  volume={Chapter 9},
  pages={Unit 9.1}
This unit describes the use of lectins for preparative glycoprotein purification. Con A-Sepharose and WGA-agarose are used for convenience and availability. Instructions are given for a small-scale pilot procedure to test for lectin binding and to determine elution conditions. There are many variations on the basic procedure in the literature, but all use the same principles: bind the protein to immobilized lectin through its sugar chain, wash away unbound protein, and elute bound protein with… CONTINUE READING