Lectin Affinity Chromatography

@article{Freeze1995LectinAC,
  title={Lectin Affinity Chromatography},
  author={Hudson H. Freeze},
  journal={Current Protocols in Protein Science},
  year={1995},
  volume={00}
}
  • H. Freeze
  • Published 1 June 1995
  • Biology
  • Current Protocols in Protein Science
This unit describes the use of lectins for preparative glycoprotein purification. Con A‐Sepharose and WGA‐agarose are used for convenience and availability. Instructions are given for a small‐scale pilot procedure to test for lectin binding and to determine elution conditions. There are many variations on the basic procedure in the literature, but all use the same principles: bind the protein to immobilized lectin through its sugar chain, wash away unbound protein, and elute bound protein with… 

COMPARATIVE STUDY OF MACROPOROUS SILICA- AND CELLULOSE-BASED SORBENTS FOR LECTIN AFFINITY CHROMATOGRAPHY

□ Preparative lectin affinity chromatography is widely used as a first step for the isolation and purification of glycoproteins. In this work lectin affinity adsorbents for preparative processes were

Affinity Chromatography: Principles and Applications

The wide applicability of this method is based on the fact that any given biomolecule that one wishes to purify usually has an inherent recognition site through which it can be bound by a natural or artificial molecule.

A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C- and O-Mannosylated Peptides.

The α-mannose-specific Burkholderia cenocepacia lectin A (BC2L-A) is used and shown to be able to retain C- and O-mannosylated peptides and might have superior properties over other mannose binding lectins for this purpose.

Affinity chromatography: a historical perspective.

Past and recent efforts in the generation of new binding agents, supports, and immobilization methods for this method are considered and various applications of affinity chromatography are summarized.

Analysis of carbohydrate residues on recombinant human thyrotropin receptor.

The data suggest that during peptide chain glycosylation, high mannose-type sugar residues are attached first and then modified by the formation of complex type structures to form the mature glycoprotein.

Characterization of a Novel Mannose-Binding Lectin with Antiviral Activities from Red Alga, Grateloupia chiangii

The mannose binding properties and tandem repeat structure of GCL may confer it the potential to act as an antiviral agent for protection against viral infection.

Purification of Organelles from Mammalian Cells

  • J. Castle
  • Biology
    Current protocols in immunology
  • 2003
The protocols in this unit emphasize different fractionation techniques that have been used for rat liver, an abundant tissue that has been a favorite of many investigators and has served as the source of many organelle preparations of excellent purity.

Lectin-Mediated Binding of Engineered Lactococcus lactis to Cancer Cells

Two lectins, B subunit of bacterial Shiga holotoxin and fungal Clitocybe nebularis lectin, are employed for surface display on the lactic acid bacterium Lactococcus lactis to see if they might serve as a carrier in targeted drug delivery when coupled to a therapeutic moiety.

Using lectins to harvest the plasma/serum glycoproteome

Combined with complementary depletion and MS technologies, lectin affinity chromatography is becoming the most widely employed method of choice for biomarker discovery in cancer and other diseases.

References

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Use of lectins in analysis of glycoconjugates.

Isolation and characterization of the two glycosylation isoforms of low molecular weight mannose 6-phosphate receptor from bovine testis. Effect of carbohydrate components on ligand binding.

Low molecular weight mannose 6-phosphate receptor from bovine testis exhibits two isoforms on sodium dodecyl sulfate-polyacrylamide gel electrophoresis that contain a common polypeptide core, but differ in their carbohydrate content.

Isolation and Characterization of the Two Glycosylation Isoforms of Low Molecular Weight Mannose 6-Phosphate Receptor from Bovine Testis

Low molecular weight mannose 6-phosphate receptor from bovine testis exhibits two isoforms on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with M, which contain a common polypeptide core, but differ in their carbohydrate content.

Phenol – sulfuric acid assay for hexoses and pentoses

  • Current Protocols in Molecular Biology
  • 1993

See above . Most current description of using lectins to characterize sugar chain structures

  • 1994