Leakage of immobilized IgG from therapeutic immunoadsorbents.

  title={Leakage of immobilized IgG from therapeutic immunoadsorbents.},
  author={Hideyuki Sato and T Kidaka and M Hori},
  journal={Applied biochemistry and biotechnology},
  volume={15 2},
In developing therapeutic immunoadsorbents (IAs), antibodies (IgG molecules) covalently immobilized on porous carriers, a leak of IgG was determined both in the storage test with buffers at 25 and 4 degrees C and in contact with plasma at room temperature (RT). The amount of antibody released from therapeutic IAs must be minimized to avoid side effects during treatment. The amount of IgG released was a. Dependent on the amount of IgG immobilized b. Much greater with CNBr-activated Sepharose 4B… CONTINUE READING