Leakage and slow allostery limit performance of single drug-sensing aptazyme molecules based on the hammerhead ribozyme.

@article{Silva2009LeakageAS,
  title={Leakage and slow allostery limit performance of single drug-sensing aptazyme molecules based on the hammerhead ribozyme.},
  author={Chamaree de Silva and Nils G Walter},
  journal={RNA},
  year={2009},
  volume={15 1},
  pages={76-84}
}
Engineered "aptazymes" fuse in vitro selected aptamers with ribozymes to create allosteric enzymes as biosensing components and artificial gene regulatory switches through ligand-induced conformational rearrangement and activation. By contrast, activating ligand is employed as an enzymatic cofactor in the only known natural aptazyme, the glmS ribozyme, which is devoid of any detectable conformational rearrangements. To better understand this difference in biosensing strategy, we monitored by… CONTINUE READING
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