Leaf callus induction and suspension culture establishment in lychee (Litchi chinensis Sonn.) cv. Huaizhi

Abstract

This study reports a protocol for leaf callus induction and suspension culture establishment in lychee cv. Huaizhi. The results showed that 12-day-old leaf explants cultured under a photoperiod of 16/8 h with their adaxial side touching the medium were the optimum conditions for leaf callus induction. Globular embryos were formed when the induced calli were… (More)
DOI: 10.1007/s11738-008-0223-x

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