Lck-dependent tyrosine phosphorylation of diacylglycerol kinase alpha regulates its membrane association in T cells.

@article{Merino2008LckdependentTP,
  title={Lck-dependent tyrosine phosphorylation of diacylglycerol kinase alpha regulates its membrane association in T cells.},
  author={Ernesto Merino and Antonia {\'A}vila-Flores and Yasuhito Shirai and Ignacio Moraga and Naoaki Saito and Isabel M{\'e}rida},
  journal={Journal of immunology},
  year={2008},
  volume={180 9},
  pages={5805-15}
}
TCR engagement triggers phospholipase Cgamma1 activation through the Lck-ZAP70-linker of activated T cell adaptor protein pathway. This leads to generation of diacylglycerol (DAG) and mobilization of intracellular Ca(2+), both essential for TCR-dependent transcriptional responses. TCR ligation also elicits transient recruitment of DAG kinase alpha (DGKalpha) to the lymphocyte plasma membrane to phosphorylate DAG, facilitating termination of DAG-regulated signals. The precise mechanisms… CONTINUE READING