Laying the Cytotaxonomic Foundations of a New Model Grass, Brachypodium distachyon (L.) Beauv.

  title={Laying the Cytotaxonomic Foundations of a New Model Grass, Brachypodium distachyon (L.) Beauv.},
  author={Robert Hasterok and John Draper and Glyn Jenkins},
  journal={Chromosome Research},
Brachypodium distachyon is a ubiquitous, temperate grass species which is being developed and exploited as an alternative model to rice, in order to gain access to important syntenic regions of the genomes of less tractable relatives such as wheat. As part of this initiative, this paper describes for the first time the cytotaxonomy of members of the polyploid series of this species, and challenges the assumption that the series evolved simply by chromosome doubling. In situ hybridization using… 
Brachypodium distachyon as a Genetic Model System.
  • E. Kellogg
  • Biology, Medicine
    Annual review of genetics
  • 2015
Brachypodium distachyon is uniquely suited for studies of floral development, vein patterning, the controls of the perennial versus annual habit, and genome organization.
Progressive refinement of the karyotyping of Brachypodium genomes.
This Tansley insight documents how key refinements in molecular cytogenetic approaches from simple fluorescence in situ hybridisation to comparative chromosome barcoding have enabled genome structure studies, and have yielded valuable information about the drivers of karyotypic reorganisation and evolution in the model grass genus Brachypodium.
Brachypodium distachyon, a New Model for the Triticeae
This chapter provides an overview of the advantages of Brachypodium as a model system and surveys the use and potential applications of this system to aid wheat, barley and Lolium research.
Brachypodium distachyon and Setaria viridis: Model Genetic Systems for the Grasses.
A historical view of the development of plant model systems is provided and several recent reports that are providing developing communities with the tools for gene discovery and pathway engineering are highlighted.
Compact genomes and complex evolution in the genus Brachypodium
Evidence is shown that B. distachyon may have a homoploid origin, involving ancestral interspecific hybridisation, although it does not appear to be a component of any of the perennial Eurasian allopolyploids.
Evolution and taxonomic split of the model grass Brachypodium distachyon.
The substantial phenotypic, cytogenetic and molecular differences detected among the three B. distachyon sensu lato cytotypes are indicative of major speciation processes within this complex that allow their taxonomic separation into three distinct species.
Analysis and Exploitation of Cereal Genomes with the Aid of Brachypodium
The biology, genetics, and genomics of Brachypodium will be reviewed as a context for the use of the plant, particularly the annual diploid B. distachyon, as a research system and current research in the plant itself on traits relevant to grain and bioenergy production will be presented.


Brachypodium distachyon. A new model system for functional genomics in grasses.
A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of "core pooid" genera that contain
Chromosome numbers in Brachypodium Beauv. (Gramineae)
The present work does not support claims made for extensive infraspecific variation in certain species and the implication of cytological knowledge for a taxonomic and evolutionary understanding of the genus is discussed.
Identification of individual chromosomes and parental genomes in Brassica juncea using GISH and FISH
Fluorescence in situ hybridization with 5S and 45S rDNA probes enabled discrimination of a substantial number of chromosomes, providing chromosomal landmarks for 20 out of 36 chromosomes of B. juncea and allowing assignment of all chromosomes to either the A or B genomes.
Molecular phylogeny of the grass genus Brachypodium P. Beauv. based on RFLP and RAPD analysis
Molecular studies confirm Brachypodium as an isolated ancient genus best placed in its own tribe (Brachypodieae).
Phylogenetic reconstruction of the genusBrachypodium P. Beauv. (Poaceae) from combined sequences of chloroplastndhF gene and nuclear ITS
A phylogenetic reconstruction of eight species of the genusBrachypodium P. Beauv. (Poaceae) has been obtained combining sequence data from the chloroplastndhF gene and the nuclear ITS, and using five
Breeding relationships in the genus Brachypodium (Poaceae: Pooideae)
Results of hybridisation experiments involving five perennial European species of Brachypodium and American perennial B. mexicanum are presented, finding some interspecific hybrids are more easily produced and are more fertile than certain intraspecific hybrids involving parents of different chromosome number.
A cereal centromeric sequence
The identification of a family of sequences located by in situ hybridisation to the centromeres of all the Triticeae chromosomes studied, including the supernumerary and midget chromosomes, the centromres of maize chromosomes and the heterochromatic regions of rice chromosomes, which will allow the evolution of the cereal centromere and their sites to be studied.
Ribosomal DNA variation and its phylogenetic implication in the genusBrachypodium (Poaceae)
The structure of ribosomal DNA of Brachypodium and several other grass species was investigated using a heterologous rDNA probe from wheat, finding restriction fragment length polymorphism and length variation of the repeat units have taxonomic value withinBrachyp sodium and are correlated with the classification of Brachypodium derived from other data.
Ribosomal DNA is an effective marker of Brassica chromosomes
Abstract  Simultaneous fluorescence in situ hybridisation with 5S and 25S rDNA probes enables the discrimination of a substantial number of chromosomes of the complement of all diploid and tetraploid
Karyotyping of Brassica amphidiploids using 5S and 25S rDNA as chromosome markers.
This paper demonstrates the use of multicolour fluorescence in situ hybridisation with 5S and 25S rDNA for karyotyping three amphidiploid species: B. napus, B. juncea and B. carinata.