Lateral resolution improvement in two-photon excitation microscopy by aperture engineering

@inproceedings{Mondal2007LateralRI,
  title={Lateral resolution improvement in two-photon excitation microscopy by aperture engineering},
  author={Partha Pratim Mondal and Alberto Diaspro},
  year={2007}
}
A technique for resolution improvement in two-photon excitation (2PE) fluorescence microscopy based on radially-symmetric annular binary filter (consist of central circular aperture and a concentric peripheral annulus) is proposed. Resolution improvement is achieved by engineering the aperture of the objective lens in a way so as to enhance high spatial frequencies. The structure of the electromagnetic field in the regions of focus and nearby regions are determined. The central lobe of the time… CONTINUE READING

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