Late proliferating and inflammatory effects on murine microvascular heart and lung endothelial cells after irradiation.

Abstract

BACKGROUND AND PURPOSE Radiotherapy of thoracic tumors increases the risk to develop cardiac diseases at later time-points. We compared time kinetics of radiation-induced changes of surface markers related to proliferation, progenitor cell development and inflammation in lung and heart microvascular endothelial cells (ECs). MATERIAL AND METHODS Mice received local thorax irradiation with a single dose of 0, 2 or 8 Gy. Following magnetic bead separation and biotin-streptavidin competition, cell surface markers of isolated ECs from the lung and heart were analyzed 5, 10, 15 and 20 weeks after irradiation by flow cytometry. RESULTS Irradiation with 8 Gy resulted in a temporary and differential up-regulation of proliferation markers (HCAM, Integrin β-3, Endoglin, VE-cadherin, VEGFR-2) on ECs. Mucosialin a progenitor marker increased in lung ECs 15-20 weeks and inflammatory markers (PECAM-1, ICAM-1, ICAM-2, VCAM-1) started to increase 10 weeks after thorax irradiation with 8 Gy. Interestingly, ICAM-1 and VCAM-1 remained up-regulated 20 weeks after irradiation in heart and lung ECs. CONCLUSIONS The persistently elevated expression density of ICAM-1 and VCAM-1 on ECs may suggest that an irradiation at 8 Gy induces late inflammatory responses in heart and lung ECs.

DOI: 10.1016/j.radonc.2015.07.029
02040608020162017
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@article{Sievert2015LatePA, title={Late proliferating and inflammatory effects on murine microvascular heart and lung endothelial cells after irradiation.}, author={Wolfgang Sievert and K J Trott and Omid Azimzadeh and Soile Tapio and Horst F. Zitzelsberger and Gabriele Multhoff}, journal={Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology}, year={2015}, volume={117 2}, pages={376-81} }