Late mitotic failure in mice lacking Sak, a polo-like kinase

  title={Late mitotic failure in mice lacking Sak, a polo-like kinase},
  author={John W Hudson and Anna Kozarova and Pierre Cheung and Jennifer C. Macmillan and Carol Jane Swallow and James C Cross and James W. Dennis},
  journal={Current Biology},

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Role for polo-like kinase 4 in mediation of cytokinesis

Inhibition of PLK4 kinase activity with a small-molecule inhibitor, CFI-400945, prevents translocation to the spindle midzone/cleavage furrow and prevents cellular abscission, leading to the generation of cells with polyploidy, increased numbers of duplicated centrosomes, and vulnerability to anaphase or mitotic catastrophe.

Polo-Like Kinase 1 Is Essential for Early Embryonic Development and Tumor Suppression

The data support that Plk1 is important for early embryonic development and may function as a haploinsufficient tumor suppressor and the loss of one PlK1 allele accelerates tumor formation.

The Sak polo-box comprises a structural domain sufficient for mitotic subcellular localization

The 2.0 Å crystal structure of a novel domain composed of the polo box motif of murine Sak is reported, showing that this domain forms homodimers both in vitro and in vivo, and localizes to centrosomes and the cleavage furrow during cytokinesis.

Requirement for the Budding Yeast Polo Kinase Cdc5 in Proper Microtubule Growth and Dynamics

Interestingly, both Cdc28 and Cdc5 were required for the proper modification of the spindle pole body components Nud1, Slk19, and Stu2 in vivo and they also phosphorylated these three proteins in vitro.

Polo-like kinases and DNA damage checkpoint: beyond the traditional mitotic functions

  • E. Bahassi
  • Biology
    Experimental biology and medicine
  • 2011
Polo-like kinases (Plks) are a family of serine–threonine kinases that play a pivotal role in cell cycle progression and in cellular response to DNA damage. The Plks are highly conserved from yeast

Polo-like kinases in cell cycle checkpoint control.

Recent studies from various eukaryotic model systems indicate that polo-like kinases (Plks) play an ever-increasing role in the regulation of cell cycle progression. Early genetic studies have

A Spindle Checkpoint Arrest and a Cytokinesis Failure by the Dominant-negative Polo-box Domain of Plk1 in U-2 OS Cells* 210

It is proposed that, in mammalian cells, the polo-box-dependent Plk1 activity is required for proper metaphase/anaphase transition and for cytokinesis.

Deficiency in Chromosome Congression by the Inhibition of Plk1 Polo Box Domain-dependent Recognition*

The predominant role of PBD-dependent binding on smooth chromosome congression at metaphase is demonstrated and the high-throughput screening system developed shows it to be essential for Plk1 localization and substrate targeting.

The novel mouse Polo-like kinase 5 responds to DNA damage and localizes in the nucleolus

The cloning of a fifth member of the polo-like kinase family named Plk5 is reported, and DNA and protein sequence analyses show that PlK5 shares more similarities with Plk2 and Plk3 than with PlK1 and PlK4.



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The polo-like kinase Plx1 prevents premature inactivation of the APCFizzy-dependent pathway in the early Xenopus cell cycle

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Polo-like kinases: a team that plays throughout mitosis.

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CDK1 Inactivation Regulates Anaphase Spindle Dynamics and Cytokinesis In Vivo

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Exit from mitosis in Drosophila syncytial embryos requires proteolysis and cyclin degradation, and is associated with localized dephosphorylation.

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Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk.

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Sak, a murine protein-serine/threonine kinase that is related to the Drosophila polo kinase and involved in cell proliferation.

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