Late events of translation initiation in bacteria: a kinetic analysis.

@article{Tomsic2000LateEO,
  title={Late events of translation initiation in bacteria: a kinetic analysis.},
  author={J Tomsic and Luca A Vitali and Tina Daviter and Andreas Savelsbergh and Roberto Spurio and P Striebeck and Wolfgang Wintermeyer and Marina V. Rodnina and Claudio O. Gualerzi},
  journal={The EMBO journal},
  year={2000},
  volume={19 9},
  pages={2127-36}
}
Binding of the 50S ribosomal subunit to the 30S initiation complex and the subsequent transition from the initiation to the elongation phase up to the synthesis of the first peptide bond represent crucial steps in the translation pathway. The reactions that characterize these transitions were analyzed by quench-flow and fluorescence stopped-flow kinetic techniques. IF2-dependent GTP hydrolysis was fast (30/s) followed by slow P(i) release from the complex (1.5/s). The latter step was rate… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 44 extracted citations

Translation initiation: structures, mechanisms and evolution.

Quarterly reviews of biophysics • 2004
View 4 Excerpts
Highly Influenced

Ribosome Structure and the Mechanism of Translation

Cell • 2002
View 4 Excerpts
Highly Influenced

Roles of helix H69 of 23S rRNA in translation initiation.

Proceedings of the National Academy of Sciences of the United States of America • 2015
View 13 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…