Late endosomes derive from early endosomes by maturation

  title={Late endosomes derive from early endosomes by maturation},
  author={Willem Stoorvogel and Ger J.A.M. Strous and Hans J. Geuze and Viola Oorschot and Alan L. Schwartzt},

Transport of residual endocytosed products into terminal lysosomes occurs slowly in rat liver endothelial cells

It is concluded that EEA1 is a useful marker for tracing early events of endocytosis in liver endothelial cells and in contrast to the rapid internalization, transit of internalized ligand through early sorting endosomes generally takes from 20 minutes to 2 hours; and exit from the late endosome is very slow, requiring several hours.

Transport from late endosomes to lysosomes, but not sorting of integral membrane proteins in endosomes, depends on the vacuolar proton pump

It is concluded that trafficking through early and late endosomes, but not to lysosome, continued upon inactivation of the vacuolar proton pump.

Delivery of ligands from sorting endosomes to late endosomes occurs by maturation of sorting endosomes

The properties of individual endosomes are studied by digital image analysis to distinguish between the two mechanisms for entry of ligands into late endOSomes and are consistent with a maturation mechanism in which the sorting endosome retains and accumulates lysosomally directed ligands until it loses its ability to fuse with newly formed endocytic vesicles and matures into a late endosom.

Maturation of early endosomes and vesicular traffic to lysosomes in relation to membrane recycling.

The controversy whether endocytic processing occurs by organellar maturation or by vesicular traffic has not been resolved. It is also not clear whether maturation continues to the stage of

Investigation of early endosomal sorting and budding

It is concluded that fusion, docking and sorting/budding, although serving different purposes, are strongly interconnected at the molecular level.

Cytoplasmic dynein-dependent vesicular transport from early to late endosomes [published erratum appears in J Cell Biol 1994 Feb;124(3):397]

The data suggest that early and late endosomes are separate, highly dynamic organelles, which are connected by a microtubule-dependent vesicular transport step, which is supported by the known role of microtubules during early toLate endosome transport in vivo.

The yeast endocytic membrane transport system

  • A. Munn
  • Biology
    Microscopy research and technique
  • 2000
The visualization of the S. cerevisiae endocytic pathway has revealed similarities toendocytic pathways visualized in higher eukaryotes, as well as new membrane trafficking pathways connecting these organelles.

The role of calcium and other ions in sorting and delivery in the late endocytic pathway.

The passage of endocytosed receptor-bound ligands and membrane proteins through the endocytic pathway of mammalian cells to lysosomes occurs via early and late endosomes. The latter contain many

Evidence for nonvectorial, retrograde transferrin trafficking in the early endosomes of HEp2 cells

A second, slower rate for Tf to leave sorting endosomes after HEp2 cells were labeled to steady state with fluorescein Tf is reported, suggesting an equilibration of Tf throughout the early endosomal system by this retrograde pathway.



Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosome

To study the fusion and separation of endocytic compartments, digital image analysis is used to quantify the accumulation of fluorescent ligands in endosomes during continuous endocytosis for periods of 1-20 min and simple mathematical models suggest that this type of iterative fractionation can lead to very high efficiency sorting.

Fusion accessibility of endocytic compartments along the recycling and lysosomal endocytic pathways in intact cells

The site of F-alpha 2M accumulation at 18 degrees C is identified as a prelysosomal late endosome that no longer fuses with newly formed endosomes since no delivery to lysosomes is observed at this temperature.

Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro

The authors' observations suggest that the spherical vesicles mediate transport between the early endosome and late endosomes and that this process requires intact microtubules.

Sorting of mannose 6-phosphate receptors and lysosomal membrane proteins in endocytic vesicles

The intracellular distributions of the cation-independent mannose 6- phosphate receptor (MPR) and a 120-kD lysosomal membrane glycoprotein (lgp120) were studied in rat hepatoma cells. Using

Intracellular fusion of sequentially formed endocytic compartments

Digital image analysis to quantify quenching in this region is found that F-Tf/AFA mixing is occurring either within this compartment or before transferrin enters it, indicating that there are some compartments which are accessible for mixing with subsequently endocytosed molecules.

The pathways of endocytosed transferrin and secretory protein are connected in the trans-Golgi reticulum

A common intracellular localization of endocytosed Tf/HRP and secretory protein could be confirmed by immuno-electron microscopy: cryosections labeled with anti-albumin as well as DAB reaction product showed double-labeling in the trans-Golgi reticulum.