Laser microdissection microscopy: application to cell culture.

@article{Mustafa2012LaserMM,
  title={Laser microdissection microscopy: application to cell culture.},
  author={Ahlam Mustafa and Cathy Cenayko and Ragai R. Mitry and Alberto Quaglia},
  journal={Methods in molecular biology},
  year={2012},
  volume={806},
  pages={
          385-92
        }
}
Laser microdissection (LMD) microscopy allows isolation of specific cell populations to target their -molecular profile. There are several different types of LMD microscopes, but they are all based on the same principle. A laser beam is used to cut out cells or tissues of interest from a histological section, cytology preparations, or live cells from tissue cultures. Live cells can be isolated using LMD and processed for downstream molecular work. RNA, DNA, and protein isolation is possible… 

Figures and Tables from this paper

Automation of single-cell techniques in neural tissue
TLDR
The work presented greatly improves the throughput of traditional single-cell methods of transfection and cell-sampling by between one and two orders of magnitude and fills in a gap in the workflow of the rapidly expanding field of single- cell analysis.
Les applications de la microdissection laser en histologie
La microdissection laser permet d’isoler des cellules, phénotypiquement identiques, à partir d’une lame de microscope portant un tissu biologique, dans l’optique de réaliser des analyses moléculaires
of Stem Cells and Genetics “ Destined to Die ” , Neural Stem and Progenitor Cells as Possible Seedbeds for Cancer : A Hypothesis and Theoretical Model 1
TLDR
The hypothesis and model presented in this article is an attempt to envisage a common mechanism through which certain cells may acquire increased apoptotic resistance and thus become ideally suited to serve as the seedbeds of cancers.
Cytochrome P450 in living donor liver transplantation
TLDR
This review focuses on recent findings regarding the biological expressions of the CYP2C19, CYP3A4, CYp3A5 and MRD1 genotypes in liver grafts before and after LDLT.
The Use of Transcriptomics to Unveil the Role of Nutrients in Mammalian Liver
  • J. Osada
  • Biology, Medicine
    ISRN nutrition
  • 2013
TLDR
In this review, the works dealing with the response of mammalian liver to different nutritional stimuli such as fasting/feeding, caloric restriction, dietary carbohydrate, cholesterol, fat, protein, bile acid, salt, vitamin, and oligoelement contents are covered.
Source and characterization of hepatic macrophages in acetaminophen‐induced acute liver failure in humans
TLDR
In AALF, the h‐mϕ population is expanded in areas of necrosis, both through proliferation of resident cells and CCL2‐dependent recruitment of circulating monocytes, which indicates that they are implicated in resolution of inflammation/tissue repair processes during AALf.
Homogeneous phenomenon of the graft when using different genotype characteristic of recipients/donors in living donor liver transplantation.
TLDR
The CYP3A5*3, MDR1-3435 and CYP 3A4*18 of the recipient could be modified by the donor so-called homogenous phenomenon when the recipient's blood drained into the liver graft.

References

SHOWING 1-3 OF 3 REFERENCES
Laser-capture microdissection.
TLDR
Laser-capture microdissection is a recently discovered state-of-the-art method to obtain cells for genetic analysis that allows capture of selected cells under direct microscopic visualization.
Culture of Animal Cells
TLDR
Culture of animal cells :, Culture ofAnimal cells : , کتابخانه دیجیتالی دانشگاه علوم پزشدکی و شهید بهشتی.