Laser microdissection in translational and clinical research

@article{Ladnyi2006LaserMI,
  title={Laser microdissection in translational and clinical research},
  author={Andr{\'a}s Lad{\'a}nyi and Ferenc Sipos and Dominika Szőke and Orsolya Galamb and B{\'e}la Moln{\'a}r and Zsolt Tulassay},
  journal={Cytometry Part A},
  year={2006},
  volume={69A}
}
Laser microdissection (LMD) is now a well established method for isolating individual cells or subcellular structures from a heterogeneous cell population. In recent years, cell, DNA, RNA, and protein based techniques has been successfully coupled to LMD and important information has been gathered through the analysis of the genome, transcriptome, and more recently the proteome of individual microdissected cells. 
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27 Citations
Background Citations
7
Methods Citations
5

27 Citations

Citation Type

Citation Type

Laser capture microdissection: methods and applications.
TLDR
Laser microdissection allows the preparation of homogenous isolates of specific subpopulations of cells from which RNA/DNA or protein can be extracted for RT-polymerase chain reaction (PCR), quantitative PCR, Western blot analyses, and mass spectrophotometry.
Laser Capture Microdissection: Insights into Methods and Applications.
TLDR
Laser capture microdissection is versatile allowing the preparation of homogenous isolates of specific subpopulations of cells from which DNA/RNA or protein can be extracted for RT-PCR, quantitative PCR, next-generation sequencing, immunoblot blot analyses, and mass spectrometry.
Laser capture microdissection: should an ultraviolet or infrared laser be used?
Combining Laser-Assisted Microdisstection with/and Immunohistochemistry- RNA Quality of Clinical LCM-Derived Samples
TLDR
Dry ice is recommended as a preserving agent for both IHC and RNA studies considering its applicability for subsequent analysis and financial costs, and major factors influencing the results are the amount and quality of material studied.
Evaluation and application of RNAs derived from laser microdissected specimens using DNA microarray for expression genomics
TLDR
Application of LCM-derived RNAs to DNA microarrays may be limited in the quantity or quality of cRNA, and may generate less hybridization potential in DNA microarray experiments, but this laser-based micro-dissection method can be applied to conduct more precise and exquisite assessment of gene expression analysis in heterogeneous tissue samples.
Laser-capture microdissection and transcriptional profiling in archival FFPE tissue in prostate cancer.
TLDR
Laser-capture microdissection (LCM) is used in isolating tumour cells in archival FFPE prostate needle biopsies and subsequent application for RNA extraction and quantitative real-time PCR (QPCR) is described.
Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling
TLDR
This protocol enables transcriptome profiling of specific cell or tissue types that are isolated from tomato using laser microdissection (LM), which has the advantage of being readily applied to any plant species without having to generate transgenic plants.
Laser capture microdissection coupled with on-column extraction LC-MS(n) enables lipidomics of fluorescently labeled Drosophila neurons.
TLDR
It is argued that this workflow represents a tremendous improvement for tissue lipidomics by integrating genetics, fluorescence microscopy, LCM and LC-MS(n) to exploit the specificity of LCM for lipidomics.
Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage
TLDR
The present study shows that the optimized LCM protocol could allow isolation of four homogenous zone-specific cell populations from MCC, meanwhile preserving RNA integrity to meet the high quality requirements for subsequent molecular analyses.
Use of laser microdissection in the analysis of renal-infiltrating T cells in MRL/lpr mice
TLDR
The results of this study suggest that the pathogenesis of renal lesions in MRL/lpr mice is complex and not due simply to the Th1 and Th2 balance, and support the concept of different molecular mechanisms for glomerulonephritis and vasculitis in these mice.
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References

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Laser-capture microdissection
TLDR
A thorough description of laser-capture microdissection techniques is provided, with an emphasis on tips and troubleshooting advice derived from LCM users.
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TLDR
This work describes a rapid noncontact method for the capture of single cells or small tissue areas of any size or shape directly within the cap of a common microfuge tube and was able to detect point mutations within codon 12 of c-Ki-ras2 mRNA after nested RT-PCR analysis.
The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis — Preliminary findings
Proteomics‐based studies offer a powerful complementary approach to DNA/RNA‐based investigations and are now being applied to investigate aspects of many diseases including cancer. However, the…
Identification of multiple mRNA and DNA sequences from small tissue samples isolated by laser-assisted microdissection.
TLDR
It is demonstrated that multiple mRNA as well as DNA sequences can be identified from a single-cell sample using a laser dissection microscope and modified nucleic acid isolation protocols.
Navigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samples
TLDR
A new LCM method called navigated LCM is described, which uses fixed but unstained tissue as starting material and thus enables us to avoid artifacts induced by tissue staining and is therefore a practical and powerful method for performing proteomic studies in specifically defined brain regions.
Gene profiling of laser-microdissected brain regions and sub-regions.
Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis: evaluation of tissue preparation and sample limitations.
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TLDR
The LCM–microSAGE approach demonstrates that large-scale expression profiles of known and unknown transcripts can be generated from pure populations of target cells obtained from human tissue samples comprised of heterogeneous mixtures of cell types.
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TLDR
It has been demonstrated that Laser Microdissection and Pressure Catapulting (LMPC) have no influence on the proliferation rate of the cells, and this new approach opens a wide field of interesting applications in cell biology, molecular pathology, and pharmacology.
Application of laser capture microdissection and proteomics in colon cancer
TLDR
A method for obtaining proteome information from laser capture microdissected tissue using colon cancer as a model and performing two dimensional gel electrophoresis and mass spectroscopic data obtained from bulk tumour samples run in parallel has been developed.
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