Laser induces apoptosis and ceramide production in human retinal pigment epithelial cells.

@article{Barak2005LaserIA,
  title={Laser induces apoptosis and ceramide production in human retinal pigment epithelial cells.},
  author={Adiel Barak and Tzipora Goldkorn and Lawrence Morse},
  journal={Investigative ophthalmology \& visual science},
  year={2005},
  volume={46 7},
  pages={
          2587-91
        }
}
PURPOSE To investigate the cellular mechanisms involved in the cell death of human retinal pigment epithelial (hRPE) cells after their exposure to laser injury. METHODS Cultured human hRPE cells were irradiated for different lengths of time and at different levels of energy using diode laser photocoagulation coupled with an intraocular laser probe. Apoptosis was determined by TUNEL staining and annexin-V labeling of phosphatidylserine exposure. Ceramide levels were quantified by the… 
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18 Citations
Background Citations
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Methods Citations
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18 Citations

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Citation Type

Photocoagulation of Human Retinal Pigment Epithelial Cells In Vitro: Evaluation of Necrosis, Apoptosis, Cell Migration, Cell Proliferation and Expression of Tissue Repairing and Cytoprotective Genes
TLDR
Using a laser system and experimental settings that comply with standards used in clinical practice, an in vitro model for in vitro photocoagulation of human RPE cells is established to isolate their contribution to the beneficial effects of laser treatment.
Real-time in vivo imaging of retinal cell apoptosis after laser exposure.
TLDR
This is the first time that DARC has been used to determine apoptotic effects in the inner nuclear layer and the ability to monitor changes spatially and temporally in vivo promises to be a major advance in the real-time assessment of retinal diseases and treatment effects.
Effects of photoelectric therapy on proliferation and apoptosis of scar cells by regulating the expression of microRNA‐206 and its related mechanisms
TLDR
Laser irradiation can significantly inhibit the proliferation of HSF cells, affect cell cycle, and increase cell apoptosis and migratory ability.
Ceramide production associated with retinal apoptosis after retinal detachment
TLDR
Retinal apoptosis during experimental retinal detachment is associated with in vivo production of ceramide and the detached retina showed intense ceramide immunostaining that was prominent in the photoreceptors, but also present to a lesser extent in other retinal layers.
Fas signaling induces raft coalescence that is blocked by cholesterol depletion in human RPE cells undergoing apoptosis.
TLDR
The cells stimulated to undergo apoptosis appear to use membrane rafts in the death-signaling process by mobilization of rafts to localized regions of the membrane that are now enriched with apoptotic signaling effectors.
In Vitro Cytological Responses against Laser Photobiomodulation for Periodontal Regeneration
TLDR
Light is shed on basic research that would ultimately lead to clinical application of periodontal phototherapy in the future through this review of the molecular biological effects of diode, Nd:YAG, Er:Y AG, Er,Cr:YSGG, and CO2 lasers irradiation on cells inperiodontal tissues.
Apoptosis in diabetic fibrovascular membranes after panretinal photocoagulation.
TLDR
The preliminary data suggest that the decrease in ambient mitogen, initiated by PRP treatment, activates apoptosis in diabetic fibrovascular membranes.
Recruitment of stem cells into the injured retina after laser injury.
TLDR
It is found that Lin(-ve) BMCs get incorporated into laser-injured retina when transplanted through either the intravitreal or intravenous route, indicating the importance of dose and route for stem cell-based treatment designed to result in retinal regeneration.
Regulating survival and development in the retina: key roles for simple sphingolipids
TLDR
Recent data support Cer and Sph as crucial mediators in the induction of photoreceptor apoptosis in diverse models of oxidative damage and neurodegeneration, and suggest that regulating their metabolism can prevent this death.
Photocoagulation of human retinal pigment epithelium in vitro: unravelling the effects on ARPE‐19 by transcriptomics and proteomics
TLDR
This study aimed at identifying the isolated effects of photocoagulation of RPE at both the mRNA and protein expression levels and confirmed its role in ischaemia and vascular leakage in retinal vascular disease.
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