Laser fluorimetry: subpicogram detection of aflatoxins using high-pressure liquid chromatography.

  title={Laser fluorimetry: subpicogram detection of aflatoxins using high-pressure liquid chromatography.},
  author={Gerald J. Diebold and Richard N. Zare},
  volume={196 4297},
The use of high-pressure liquid chromatographic separation in conjunction with laser-induced fluorescence detection permits the analysis of trace fluorescent species at new limits of sensitivity. This technique was applied to the carcinogens aflatoxins B1, B2, G1, and G2, which were linearly quantitated to 7.5 x 10(-13) gram. The procedure consists of forming more fluorescent aflatoxin derivatives, eluting the aflatoxins from a reverse-phase column, focusing the 325-nanometer output of a helium… Expand
Laser fluorimetry for capillary column liquid chromatography: high-sensitivity detection of derivatized biological compounds.
A high-sensitivity, low-dead-volume laser-induced fluorescence detector has been designed for use in capillary column liquid chromatography. This detector exhibits femtogram detection limits and aExpand
Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection.
  • W. Kok
  • Chemistry, Medicine
  • Journal of chromatography. B, Biomedical applications
  • 1994
Various derivatization methods for the fluorometric detection of aflatoxins after separation by HPLC are reviewed and the methods are compared with respect to experimental convenience, selectivity, reproducibility and suitability for automation. Expand
Laser Fluorescence Detection in Microcolumn Liquid Chromatography: Application to Derivatized Carboxylic Acids
A laser-induced fluorescence (LIF) detector is evaluated for use with high-efficiency liquid chromatography columns of capillary dimensions. This detector employs a cw helium-cadmium laser (325 nm,Expand
Three-dimensional high-performance liquid chromatography based on time-resolved laser fluorimetry
Abstract Microcolumn high-performance liquid chromatography is used for trace determinations of polynuclear aromatic hydrocarbons. The detector is a laser fluorimetric system with subnanosecond timeExpand
Determination of zearalenone in corn by laser fluorimetry.
By combining laser fluorimetry with high pressure liquid chromatography, this work is able to detect and quantitate the naturally fluorescent mycotoxin, zearalenone, in contaminated corn samples and places a limit of 5 ppb on the detection of zearAlenone by this procedure. Expand
Time-resolved fluorometry in detection of ultratrace polycyclic aromatic hydrocarbons in lake waters by liquid chromatography
For determination of polycyclic aromatic hydrocarbons in natural lake waters at ultratrace levels, a time-resolved fluorescence measurement system was developed. By replacing the Xe lamp of aExpand
Time-resolved laser-induced fluorescence with high-performance liquid chromatography for analysis of polycyclic aromatic hydrocarbon mixtures
Abstract Time-resolved fluorescence is used to enhance the selectivity for polycyclic aromatic hydrocarbon mixtures by high-performance liquid chromatography. A pulsed laser is used as the excitationExpand
Laser-induced fluorescence detection of native-fluorescent analytes in column liquid chromatography, a critical evaluation
Abstract This paper critically evaluates aspects that need to be considered when applying lasers instead of conventional lamps in fluorescence detection combined with column liquid chromatographyExpand
High-performance liquid chromatographic detector based on near-infrared semiconductor laser fluorimetry
Abstract Three polymethine dyes, which fluoresce at 780–820 nm, are separated by reversed-phase chromatography and detected fluorimetrically with a semiconductor laser for excitation. The detectionExpand
Factors influencing performance in the rapid separation of aflatoxins by micellar electrokinetic capillary chromatography.
Small (25 mum) internal diameter capillaries are found to be critical in maintaining high efficiency under rapid MECC separation conditions, and considerations important in the analysis of aflatoxins in real matrices are presented. Expand


Comparison of methods for aflatoxin analysis by high-pressure liquid chromatography.
  • L. M. Seitz
  • Chemistry, Medicine
  • Journal of chromatography
  • 1975
Reversed-phase columns packed with octadecyl and phenyl reversed phases did not provide adequate separation of aflatoxins. A peculiar adsorption column provided partial separation, i.e. B1 and B2Expand
The fluorescence spectra and fluorescence quantum efficiencies of aflatoxins in solvents of different polaraties are presented and it is shown that the solvent greatly influences the fluorescence of a flatoxins. Expand
Formation of aflatoxin derivatives on thin layer chromatographic plates.
  • W. Przyblylski
  • Chemistry, Medicine
  • Journal - Association of Official Analytical Chemists
  • 1975
Rapid confirmation of the presence of aflatoxins B-1 and G-1 in foods is provided by reaction with trifluoroacetic acid at the origin of a thin layer chromatographic plate. The procedure has beenExpand
Aflatoxin separation by high-pressure liquid chromatography.
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  • Chemistry, Medicine
  • Journal of chromatography
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