Laser fluorimetry: subpicogram detection of aflatoxins using high-pressure liquid chromatography.

@article{Diebold1977LaserFS,
  title={Laser fluorimetry: subpicogram detection of aflatoxins using high-pressure liquid chromatography.},
  author={G. Diebold and R. Zare},
  journal={Science},
  year={1977},
  volume={196 4297},
  pages={
          1439-41
        }
}
The use of high-pressure liquid chromatographic separation in conjunction with laser-induced fluorescence detection permits the analysis of trace fluorescent species at new limits of sensitivity. This technique was applied to the carcinogens aflatoxins B1, B2, G1, and G2, which were linearly quantitated to 7.5 x 10(-13) gram. The procedure consists of forming more fluorescent aflatoxin derivatives, eluting the aflatoxins from a reverse-phase column, focusing the 325-nanometer output of a helium… Expand
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