Laser-evoked synaptic transmission in cultured hippocampal neurons expressing channelrhodopsin-2 delivered by adeno-associated virus

@article{Wang2009LaserevokedST,
  title={Laser-evoked synaptic transmission in cultured hippocampal neurons expressing channelrhodopsin-2 delivered by adeno-associated virus},
  author={Jennifer Wang and Mazahir T. Hasan and H. Sebastian Seung},
  journal={Journal of Neuroscience Methods},
  year={2009},
  volume={183},
  pages={165-175}
}
We present a method for studying synaptic transmission in mass cultures of dissociated hippocampal neurons based on patch clamp recording combined with laser stimulation of neurons expressing channelrhodopsin-2 (ChR2). Our goal was to use the high spatial resolution of laser illumination to come as close as possible to the ideal of identifying monosynaptically coupled pairs of neurons, which is conventionally done using microisland rather than mass cultures. Using recombinant adeno-associated… CONTINUE READING

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