Laser-assisted microdissection for real-time PCR sample preparation.

  title={Laser-assisted microdissection for real-time PCR sample preparation.},
  author={Pamela Pinzani and C. James Orlando and Mario Pazzagli},
  journal={Molecular aspects of medicine},
  volume={27 2-3},
Laser-assisted microdissection (LMD) has been developed to procure precisely the cells of interest in a tissue specimen, in a rapid and practical manner. Together with real-time PCR and RT-PCR techniques, it is now feasible to study genetic alterations, gene expression features and proteins in defined cell populations from complex normal and diseased tissues. The process that brings from sample collection to the final quantitative results is articulated in several steps, each of which requires… CONTINUE READING

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