Laser-UV-microirradiation of Chinese hamster cells: the influence of the distribution of photolesions on unscheduled DNA synthesis.

@article{Cremer1981LaserUVmicroirradiationOC,
  title={Laser-UV-microirradiation of Chinese hamster cells: the influence of the distribution of photolesions on unscheduled DNA synthesis.},
  author={Christoph Cremer and Thomas Cremer and G. Jabbur},
  journal={Photochemistry and photobiology},
  year={1981},
  volume={33 6},
  pages={
          925-8
        }
}
— Fibroblastoid Chinese hamster cells synchronized by mitotic selection were microirradiated in G1, using a low power laser-UV-microbeam (λ= 257 nm). The incident energy was either concentrated on a small part of the nucleus (mode 1) or distributed over the whole nucleus (mode 11). Using the same incident UV energy, the local UV fluences were estimated to differ by two orders of magnitude. Following microirradiation the cells were incubated with [3H]-thymidine for 2 h and thereafter processed… CONTINUE READING