Laser Microdissection and Spatiotemporal Pinoresinol-Lariciresinol Reductase Gene Expression Assign the Cell Layer-Specific Accumulation of Secoisolariciresinol Diglucoside in Flaxseed Coats

@inproceedings{Fang2016LaserMA,
  title={Laser Microdissection and Spatiotemporal Pinoresinol-Lariciresinol Reductase Gene Expression Assign the Cell Layer-Specific Accumulation of Secoisolariciresinol Diglucoside in Flaxseed Coats},
  author={Jingjing Fang and A{\"i}na Ramsay and Sullivan Renouard and Christophe Hano and Fr{\'e}d{\'e}ric Lamblin and Brigitte Chabbert and François Mesnard and Bernd Schneider},
  booktitle={Front. Plant Sci.},
  year={2016}
}
The concentration of secoisolariciresinol diglucoside (SDG) found in flaxseed (Linum usitatissimum L.) is higher than that found in any other plant. It exists in flaxseed coats as an SDG-3-hydroxy-3-methylglutaric acid oligomer complex. A laser microdissection method was applied to harvest material from different cell layers of seed coats of mature and developing flaxseed to detect the cell-layer specific localization of SDG in flaxseed; NMR and HPLC were used to identify and quantify SDG in… CONTINUE READING
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