Larval Tenebrio molitor (Coleoptera: Tenebrionidae) Fat Body Extracts Catalyze Firefly D‐Luciferin‐ and ATP‐Dependent Chemiluminescence: A Luciferase‐like Enzyme *

@article{Viviani1996LarvalTM,
  title={Larval Tenebrio molitor (Coleoptera: Tenebrionidae) Fat Body Extracts Catalyze Firefly D‐Luciferin‐ and ATP‐Dependent Chemiluminescence: A Luciferase‐like Enzyme *},
  author={Vadim R Viviani and Eteivino J. H. Bechara},
  journal={Photochemistry and Photobiology},
  year={1996},
  volume={63}
}
Ultraweak light emission was detected upon injection of firefly luciferin into live Tenebrio larvae. A chemilumi‐nescent enzymatic activity dependent on molecular oxygen, D‐luciferin and MgATP was then isolated from larval fat body extracts by precipitation with 70% ammonium sulfate. D‐Luciferin and ATP can be replaced by luciferyl‐adenylate. Pyrophosphate is a main product from the chemiluminescent reaction. The in vitro chemiluminescence intensity was not affected by peroxidase inhibitors… Expand
The origin of luciferase activity in Zophobas mealworm AMP/CoA-ligase (protoluciferase): luciferin stereoselectivity as a switch for the oxygenase activity.
  • V. Viviani, V. Scorsato, +4 authors J. Barbosa
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  • 2010
TLDR
Results suggest that stereoselectivity for D-luciferin may have been a key event for the origin of oxygenase/lucifierase activity in AMP/CoA-ligases, and that efficient luciferase activity may have further evolved mainly by increasing the catalytic constant of the oxidative reaction and the quantum yield of bioluminescence. Expand
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TLDR
Data suggest that in elaterids, bioluminescence and reactions catalyzed by antioxidant enzymes may cooperate to minimize oxidative stress. Expand
Structural evolution of luciferase activity in Zophobas mealworm AMP/CoA-ligase (protoluciferase) through site-directed mutagenesis of the luciferin binding site.
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  • 2011
TLDR
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TLDR
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This review focuses on chemical and biological aspects of Brazilian luminescent beetles (Coleoptera) belonging to the Lampyridae (fireflies), Elateridae (click-beetles), and Phengodidae (railroad-worms) families. Expand
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