Large-scale purification of membrane-containing bacteriophage PRD1 and its subviral particles.

@article{Bamford1991LargescalePO,
  title={Large-scale purification of membrane-containing bacteriophage PRD1 and its subviral particles.},
  author={Jaana K. H. Bamford and Dennis H Bamford},
  journal={Virology},
  year={1991},
  volume={181 1},
  pages={
          348-52
        }
}
PRD1 is a dsDNA virus that infects Escherichia coli and Salmonella typhimurium. The genome is a linear molecule with 5' covalently linked terminal protein. The virus has a lipid membrane inside the protein coat. We describe the large-scale purification of the virus using a zonal rotor and the yields and quality of the virus and its subviral assemblies for subsequent biophysical measurements. 
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