Large-scale purification of latex bead phagosomes from mouse macrophage cell lines and subsequent preparation for high-throughput quantitative proteomics.

@article{Rupper2008LargescalePO,
  title={Large-scale purification of latex bead phagosomes from mouse macrophage cell lines and subsequent preparation for high-throughput quantitative proteomics.},
  author={Adam C Rupper and James A. Cardelli},
  journal={Methods in molecular biology},
  year={2008},
  volume={445},
  pages={
          339-51
        }
}
Phagocytosis involves the engagement of a diverse array of cell surface receptors whose signal must be integrated on the membrane of the forming phagosomal cup. This method enables the quantitative proteomic analysis of phagosome fractions derived from phagocytes stimulated under two different conditions, thus allowing the complexity of phagosomal signaling to be analyzed in terms of the quantitative changes in phagosomal fraction protein content. 
BETA

Similar Papers

Topics from this paper.

Citations

Publications citing this paper.