Large-scale purification of human islets utilizing discontinuous albumin gradient on IBM 2991 cell separator.

@article{Lake1989LargescalePO,
  title={Large-scale purification of human islets utilizing discontinuous albumin gradient on IBM 2991 cell separator.},
  author={Stephen P. Lake and P D Bassett and Audrey P. Larkins and J Revell and Krzysztof Walczak and Jeffrey Chamberlain and G M Rumford and Nicholas J. M. London and Peter S Veitch and Peter R. F. Bell},
  journal={Diabetes},
  year={1989},
  volume={38 Suppl 1},
  pages={143-5}
}
A new method is described for the large-scale purification of human pancreatic islets with a discontinuous gradient of bovine serum albumin formed on an IBM 2991 cell separator. Fifteen human pancreases were processed, and after density-gradient centrifugation, a mean of 2643 islets/ml pancreatic digest were recovered with a mean purity of 63% and contained in 430 microliter mean vol. Viability of gradient-isolated islets was compared with that of non-density-gradient islets (handpicked) and… CONTINUE READING

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