Large-scale purification of human BACE expressed in mammalian cells and removal of the prosegment with HIV-1 protease to improve crystal diffraction.

Abstract

BACE, or beta-secretase, is an attractive target in the treatment of Alzheimer's Disease because of its involvement in the generation of amyloid beta peptides. BACE is a type I transmembrane aspartyl protease composed of pre-, pro-, catalytic, transmembrane and cytoplasmic domains. For the present study, the coding sequence was truncated just before the… (More)

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@article{Emmons2008LargescalePO, title={Large-scale purification of human BACE expressed in mammalian cells and removal of the prosegment with HIV-1 protease to improve crystal diffraction.}, author={Thomas L Emmons and Mary E Shuck and Merrill S. Babcock and John S. Holloway and Joseph Leone and Jim Durbin and Donna J Paddock and Denise B Prince and Robert L Heinrikson and H David Fischer and Michael J Bienkowski and Timothy E. Benson and Alfredo G Tomasselli}, journal={Protein and peptide letters}, year={2008}, volume={15 2}, pages={119-30} }