Large-scale purification and some properties of the mitochondrial aspartate aminotransferase from pig heart.

@article{Barra1976LargescalePA,
  title={Large-scale purification and some properties of the mitochondrial aspartate aminotransferase from pig heart.},
  author={Donatella Barra and Francesco Bossa and Shawn Doonan and Hussein Mahmoud Fahmy and Filippo Martini and Graham J. Hughes},
  journal={European journal of biochemistry},
  year={1976},
  volume={64 2},
  pages={
          519-26
        }
}
A method has been developed which allows isolation of 0.3--0.5 g of mitochondrial aspartate aminotransferase in five days starting from 10 pig hearts; the method does not involve initial preparation of mitochondria. Mitochondrial malate dehydrogenase and the cytoplasmic aspartate aminotransferase may conveniently be recovered from side fractions. The product mitochondrial aspartate aminotransferase is homogeneous as judged by various electrophoretic techniques and by N-terminal analysis… CONTINUE READING