Large scale production of d-lactate dehydrogenase for the stereospecific reduction of pyruvate and phenylpyruvate

@article{Hummel2004LargeSP,
  title={Large scale production of d-lactate dehydrogenase for the stereospecific reduction of pyruvate and phenylpyruvate},
  author={Werner Hummel and Horst Robert Sch{\"u}tte and M. -R. Kula},
  journal={European journal of applied microbiology and biotechnology},
  year={2004},
  volume={18},
  pages={75-85}
}
To initiate studies of the stereospecific reduction of pyruvate and phenylpyruvate to the corresponding d-2-hydroxyacids a limited screening was carried out for microorganisms possessing a high NADH-dependet d-lactate dehydrogenase activity. Lactobacillus confusus was found to produce the desired dehydrogenase, which showed also relatively high activity towards phenylpyruvate, so this strain was selected for large scale production of the enzyme. A procedure for large scale purification of the… CONTINUE READING