Large-scale production of coenzyme F420-5,6 by using Mycobacterium smegmatis.

@article{Isabelle2002LargescalePO,
  title={Large-scale production of coenzyme F420-5,6 by using Mycobacterium smegmatis.},
  author={Dale W. Isabelle and D Randall Simpson and Lacy Daniels},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 11},
  pages={5750-5}
}
Production of coenzyme F420 and its biosynthetic precursor FO was examined with a variety of aerobic actinomycetes to identify an improved source for these materials. Based on fermentation costs, safety, and ease of growth, Mycobacterium smegmatis was the best source for F420-5,6. M. smegmatis produced 1 to 3 micromol of intracellular F420 per liter of culture, which was more than the 0.85 to 1.0 micromol of F420-2 per liter usually obtained with Methanobacterium thermoautotrophicum and… CONTINUE READING

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