Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp

@inproceedings{Thammasorn2015LargescalePA,
  title={Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp},
  author={Thitiporn Thammasorn and Pakkakul Sangsuriya and Watcharachai Meemetta and Saengchan Senapin and Sarocha Jitrakorn and Triwit Rattanarojpong and Vanvimon Saksmerprome},
  booktitle={BMC biotechnology},
  year={2015}
}
BackgroundRNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA targeting the viral gene. In this study, we employed a combinatorial approach to interfere multiple gene functions of white spot syndrome virus (WSSV), the most lethal shrimp virus, using a single-batch of dsRNA, so-called “multi-WSSV dsRNA.” A co-cultivation of RNase-deficient E. coli was developed to produce dsRNA targeting a major structural protein… CONTINUE READING
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