Large-scale preparation of biologically active measles virus haemagglutinin expressed by attenuated vaccinia virus vectors.

@article{Kidokoro2002LargescalePO,
  title={Large-scale preparation of biologically active measles virus haemagglutinin expressed by attenuated vaccinia virus vectors.},
  author={Minoru Kidokoro and Atsuko Aoki and Kiyoshi Horiuchi and Hisatoshi Shida},
  journal={Microbes and infection},
  year={2002},
  volume={4 10},
  pages={1035-44}
}
A procedure described here allows the efficient and rapid purification of histidine-tagged measles virus haemagglutinin that is synthesized under the control of powerful promoters (PSFJ1-10 and PSFJ2-16) of the highly attenuated vaccinia virus (VV) strain LC16mO. A single affinity chromatography step purifies recombinant haemagglutinin proteins from the lysates of cells infected with the recombinant VVs. The recovery and purity are both very high (a yield of 0.5-2.8 mg/10(8) cells and purity of… CONTINUE READING

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