Large-scale preparation and in vitro characterization of biologically active human placental (20 and 22K) and pituitary (20K) growth hormones: placental growth hormones have no lactogenic activity in humans.

@article{Solomon2006LargescalePA,
  title={Large-scale preparation and in vitro characterization of biologically active human placental (20 and 22K) and pituitary (20K) growth hormones: placental growth hormones have no lactogenic activity in humans.},
  author={Gili Solomon and Shay Reicher and Eugene E Gussakovsky and J-Baptiste Jomain and Arieh Gertler},
  journal={Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society},
  year={2006},
  volume={16 5-6},
  pages={297-307}
}
Expression plasmids containing DNA sequences optimized for expression in Escherichia coli were prepared encoding human pituitary (hGH-N 20K) and placental (hGH-V 20 and 22K) growth hormones. The proteins were expressed in bacteria, refolded and purified to homogeneity by anion-exchange chromatography on Q-Sepharose according to a unique protocol developed for each protein. The yields from 5l of fermentation culture varied between 400 and 700mg of electrophoretically pure, over 95% monomeric… CONTINUE READING

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