Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.

@article{Tawada2002LargescalePP,
  title={Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.},
  author={Akira Tawada and Takahiro Masa and Yoji Oonuki and Atsushi Watanabe and Yuji Matsuzaki and Akira A Asari},
  journal={Glycobiology},
  year={2002},
  volume={12 7},
  pages={421-6}
}
Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by using HPLC analyses, FACE, and ESI-MS. (1)H and (13)C NMR assignments and elemental analyses were obtained for each HA oligosaccharide. Endotoxins, proteins, and DNA were absent or in trace amounts in these… CONTINUE READING

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