Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes

  title={Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes},
  author={Ylva Ivarsson and Roland Arnold and Megan E. McLaughlin and Satra Nim and Rakesh Joshi and Debashish Ray and Bernard A. Liu and Joan Teyra and Tony Pawson and Jason Moffat and Shawn S.-C. Li and Sachdev S. Sidhu and Philip M. Kim},
  journal={Proceedings of the National Academy of Sciences},
  pages={2542 - 2547}
Significance Although knowledge about the human interactome is increasing in coverage because of the development of high-throughput technologies, fundamental gaps remain. In particular, interactions mediated by short linear motifs are of great importance for signaling, but systematic experimental approaches for their detection are missing. We fill this important gap by developing a dedicated approach that combines bioinformatics, custom oligonucleotide arrays and peptide-phage display. We… 

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